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Interruption of Ang1:Tie2 binding assay

Figure 1. Titration of Ang1 in the presence of different fixed amount of Tie2-Fc. Kd for Ang1 and Tie2 binding is  between 1 to 2 nM under all three different Tie2-Fc concentrations.



Figure 2.  Evaluation of both positive control and negative control in the assay. The detection limit of the assay is around 1 nM.



Kits Components:       


-Pair of antibodies

-Positive control

-Negative control


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