New Era Pharma: "Ideas to Molecules"

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Interruption of human B7:CTLA4 binding assay


 

Figure 1. Titration of human CTLA4 in the presence of different fixed amount of human B7-1. Apparent Kd for hCTLA4 and hB7-1 binding is  between 0.8 nM.


 

 

Figure 2.  Evaluation of both positive control and negative control in the assay. The anti-CTLA4 antibody demonstrated an IC50 of ~20nM.


 

Kits Components:       

 

-Pair of antibodies

-Positive control

-Negative control

 

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