New Era Pharma: "Ideas to Molecules"

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Interruption of hB7-1:CD28 binding assay



Figure 1. Titration of human CD28 in the presence of different fixed amount of human B7-1. Apparent Kd for hCD28 and hB7-1 binding is  7 nM.


 

Figure 2.  Evaluation of both positive control and negative control in the assay. The anti-CD antibody demonstrated an IC50 of ~4nM.

 

 

Kits Components:       

 

-Pair of antibodies

-Positive control

-Negative control

 

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