Interruption of mDLL4:hNotch1 binding assay
Figure 1. Since no mouse Notch-1 protein available, human and rat Notch-1 protein are used here. Titration of mouse hDLL4 in the presence of different fixed amount of hNotch-1 or rat Notch-1 . Apparent Kd for hNotch1 or rat Notch-1 and hDLL4 binding is around 5 nM.
Figure 2. No commercially available positive controls available. Direct evaluation of compounds resulted in positive leads. Human Notch-1 is used in this experiment and the use of rat Notch-1 resulted in similar results.
-Pair of antibodies
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